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celltrace violet ctv  (Thermo Fisher)


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    Structured Review

    Thermo Fisher celltrace violet ctv
    Celltrace Violet Ctv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+violet+ctv/celltrace+violet/pmc10362402__mmc2-242-9-17
    Average 90 stars, based on 1 article reviews
    celltrace violet ctv - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Sustained mTORC1 activation in activated T cells impairs vaccine responses in older individuals.
    Article Snippet: .. PBMCs were labeled with CellTrace Violet (CTV; Thermo Fisher Scientific) and cultured with the peptide megapool (1 μg/ml) in RPMI 1640 media (Sigma- Aldrich) supplemented with 5% human AB serum (Sigma- Aldrich), anti- CD28 (1 μg/ml; eBioscience) and penicillin and streptomycin (100 U/ml; Thermo Fisher Scientific). ..

    Article Title: Sustained mTORC1 activation in activated T cells impairs vaccine responses in older individuals
    Article Snippet: .. PBMCs were labeled with CellTrace Violet (CTV; Thermo Fisher Scientific) and cultured with the peptide megapool (1 μg/ml) in RPMI 1640 media (Sigma-Aldrich) supplemented with 5% human AB serum (Sigma-Aldrich), anti-CD28 (1 μg/ml; eBioscience) and penicillin and streptomycin (100 U/ml; Thermo Fisher Scientific). ..

    Article Title: Initial or continuous coculture with umbilical cord-derived mesenchymal stromal cells facilitates in vitro expansion of human regulatory T-cell subpopulations.
    Article Snippet: .. Cultureexpanded Tregs were prestained with CellTrace Far Red (CTFR, Thermo Fisher Scientific), while Teffs were prestained with CellTrace Violet (CTV, Thermo Fisher Scientific) using labeling protocols that were optimized for fluorescence intensity and cell viability. ..

    Article Title: Dual targeting of CD19 and CD22 against B-ALL using a novel high-sensitivity aCD22 CAR
    Article Snippet: 31 No 7 July 2023 2101 Molecular Therapy BioLegend Deluxe kits (431806 and 430106, BioLegend) according to the manufacturer’s instructions. .. Proliferation assay and tonic signaling PBMCs were labeled with CellTrace Violet (CTV) according to manufacturer’s instructions (C34557, ThermoFisher Scientific) and cultured with 0.5 105 target cells at a 1:1 E:T ratio for 96 h. The proliferation was determined as CD3+ sCD22+ transduced T cell number and percentage of CTV% decrease. ..

    Article Title: Dual IL-2/IL-21 priming augments the anti-tumor function of CD16V-CAR/TRAIL-engineered NK cells in combination with monoclonal antibodies.
    Article Snippet: .. Target tumor cells were labeled with 1 μM CellTrace Violet (CTV; Thermo Fisher Scientific, USA), seeded into 24-well plates at 2.5 × 105 cells per well, and allowed to adhere overnight. ..

    Cell Culture:

    Article Title: Sustained mTORC1 activation in activated T cells impairs vaccine responses in older individuals.
    Article Snippet: .. PBMCs were labeled with CellTrace Violet (CTV; Thermo Fisher Scientific) and cultured with the peptide megapool (1 μg/ml) in RPMI 1640 media (Sigma- Aldrich) supplemented with 5% human AB serum (Sigma- Aldrich), anti- CD28 (1 μg/ml; eBioscience) and penicillin and streptomycin (100 U/ml; Thermo Fisher Scientific). ..

    Article Title: Sustained mTORC1 activation in activated T cells impairs vaccine responses in older individuals
    Article Snippet: .. PBMCs were labeled with CellTrace Violet (CTV; Thermo Fisher Scientific) and cultured with the peptide megapool (1 μg/ml) in RPMI 1640 media (Sigma-Aldrich) supplemented with 5% human AB serum (Sigma-Aldrich), anti-CD28 (1 μg/ml; eBioscience) and penicillin and streptomycin (100 U/ml; Thermo Fisher Scientific). ..

    Article Title: Dual targeting of CD19 and CD22 against B-ALL using a novel high-sensitivity aCD22 CAR
    Article Snippet: 31 No 7 July 2023 2101 Molecular Therapy BioLegend Deluxe kits (431806 and 430106, BioLegend) according to the manufacturer’s instructions. .. Proliferation assay and tonic signaling PBMCs were labeled with CellTrace Violet (CTV) according to manufacturer’s instructions (C34557, ThermoFisher Scientific) and cultured with 0.5 105 target cells at a 1:1 E:T ratio for 96 h. The proliferation was determined as CD3+ sCD22+ transduced T cell number and percentage of CTV% decrease. ..

    Proliferation Assay:

    Article Title: Tebentafusp elicits on-target cutaneous immune responses driven by cytotoxic T-cells in uveal melanoma patients
    Article Snippet: .. 610 T-cell proliferation assay: Pan T-cells were pre-stained with 2.5 μM CellTrace Violet (CTV, 611 ThermoFisher, UK) according to the manufacturer’s guidelines. ..

    Article Title: Galectin-3 regulates erythropoiesis and enhances the immunoregulatory properties of CD71+ erythroid cells across developmental stages
    Article Snippet: .. Proliferation assay was conducted using CellTrace Violet (CTV, Thermo Fisher Scientific) according to our previous methods. .. Paraformaldehyde-fixed cells were acquired using a BD LSR Fortessa-SORP or Fortessa-X20 (BD Bioscience).

    Article Title: Dual targeting of CD19 and CD22 against B-ALL using a novel high-sensitivity aCD22 CAR
    Article Snippet: 31 No 7 July 2023 2101 Molecular Therapy BioLegend Deluxe kits (431806 and 430106, BioLegend) according to the manufacturer’s instructions. .. Proliferation assay and tonic signaling PBMCs were labeled with CellTrace Violet (CTV) according to manufacturer’s instructions (C34557, ThermoFisher Scientific) and cultured with 0.5 105 target cells at a 1:1 E:T ratio for 96 h. The proliferation was determined as CD3+ sCD22+ transduced T cell number and percentage of CTV% decrease. ..

    Fluorescence:

    Article Title: Initial or continuous coculture with umbilical cord-derived mesenchymal stromal cells facilitates in vitro expansion of human regulatory T-cell subpopulations.
    Article Snippet: .. Cultureexpanded Tregs were prestained with CellTrace Far Red (CTFR, Thermo Fisher Scientific), while Teffs were prestained with CellTrace Violet (CTV, Thermo Fisher Scientific) using labeling protocols that were optimized for fluorescence intensity and cell viability. ..

    other:

    Article Title: Supporting Information for Somatic Mutations in Tumor-Infiltrating Lymphocytes Impact on Antitumor Immunity
    Article Snippet: ImageJ software version 1.53k (Wayne Rasband, NIH, Bethesda, MD) was used for quantification.



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    Image Search Results


    Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Journal: Drug Delivery

    Article Title: Selective expansion and differentiation of antigen-specific CD4 + T-helper cells by engineered extracellular vesicles

    doi: 10.1080/10717544.2025.2509969

    Figure Lengend Snippet: Selective expansion and differentiation of T cells by AP-EVs-Th1 and AP-EVs-Th2 in vitro . (a) Lymph node T cells from C57BL/6 and OT-II transgenic mice were combined in a 1:1 ratio. The combined cells were labeled with CellTrace Violet (CTV) and treated with control EVs or AP-EVs-Th1. (b) The combined cells were co-cultured with AP-EVs-Th1, control EVs, or anti-mouse CD3/CD28 conjugated beads for three days, and proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (c) Flow cytometric plots of CTV-labeled OT-II (CD45.2/CD45.1) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th1, or control EVs. (d) Percentage of T-bet-expressing CD4 + T cells. (e) Bar graph showing the percentage of T-bet-expressing wild-type (WT) CD4 + T or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th1 (orange). (f) The combined cells were co-cultured with AP-EVs-Th2 or control EVs for three days, and the proliferation and differentiation of OT-II T cells were analyzed via flow cytometry. (g) Flow cytometric plots of CTV-labeled OT-II (CD45.2) and polyclonal (CD45.1) T cells cultured with anti-mouse CD3/CD28 conjugated beads, AP-EVs-Th2, or control EVs. (h) Percentage of GATA-3-expressing CD4 + T cells. (i) Bar graph showing the percentage of GATA-3-expressing WT CD4 + or OT-II T cells under different concentrations of control EVs (black) and AP-EVs-Th2 (orange).

    Article Snippet: Lymph node T cells isolated from C57BL/6 and OT-II transgenic mice were stained with 1 μM CellTrace Violet (CTV) Cell Proliferation Kit (Thermo Fisher Scientific, Waltham, MA) for flow cytometry and incubated at 37 °C for 3 min.

    Techniques: In Vitro, Transgenic Assay, Labeling, Control, Cell Culture, Flow Cytometry, Expressing